Plant Biotechnology 24, 255–259 (2007)
نویسندگان
چکیده
The oligosaccharide structures of total glycoproteins in suspension-cultured Arabidopsis thaliana MM2d cells are determined. The N-linked sugar chains released by hydrazinolysis were labeled with 2-aminopyridine, and analyzed by a combination of HPLC, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, and exoglucosidase digestion. The glycan structures determined were GlcNAcMan3FucXylGlcNAc2 (8.5%), Man3FucXylGlcNAc2 (70.0%), Man3XylGlcNAc2 (13.7%), and GlcNAcMan3XylGlcNAc2 (7.8%). All the glycan structures of glycoproteins from Arabidopsis MM2d cells used in this study contain typical plant bisecting either b1,2-xylose or a1,3-fucose residues, showing that suspension-cultured MM2d cells have a glycosylation potential similar to that of tobacco BY2 suspensioncultured cells.
منابع مشابه
Pharmacological Reviews
ACTH “big ACTH”, 175 “little ACTH”, 175 Acetyl strophanthidin, radioimmunoassay, 221 Adenylate cyclase activity, measurement by radioimmunoas8ay, 312 beta-Adrenergic binding sites catecholamine assay vehicle, 259 heart muscle membrane fractions, 259 Adrenergic receptors, current concepts, 259 alpha-Adrenergic receptors, catecholamines, 261 beta-Adrenergic receptors, catecholamines, 261 Aldoster...
متن کاملPlant Biotechnology 24, 237–240 (2007)
EARLY FLOWERING 3 (ELF3) plays key roles in the control of circadian rhythms, photoperiodic flowering, hypocotyls length and response to light in Arabidopsis. ELF3, however, encodes a protein without any motifs or domains of known function. Biochemical function of ELF3 has been largely unknown. Here we show the first demonstration of diversity of numbers of polyglutamines in the ELF3 among 60 A...
متن کاملPlant Biotechnology 24, 339–344 (2007)
Cytosines in eukaryotic DNA is often methylated to yield 5-methylcytosines (mC), which play an important role in controlling gene expression. This is referred as DNA methylation, and its status dynamically changes during plant growth by active methylation and demethylation. DNA glycosylases have been known to possess base excision DNA repair activity, and an Arabidopsis enzyme, ROS1 (Repressor ...
متن کاملRNAi for revealing and engineering plant gene functions.
RNA interference (RNAi) is now widely used in plant biotechnology, both as a useful tool for discovering or validating gene functions as well as a quick way of engineering specific reductions in expression of chosen genes. Although the amazing popularity of RNAi as a biotechnology tool is certainly justified, the underlying biology is still being worked out and the relative advantages and disad...
متن کاملPlant Biotechnology 24, 17–25 (2007)
Tomato (Solanum lycopersicum) is a model plant of the Solanaceae family. Various biological aspects of tomato have been investigated with molecular biological approaches, and a significant amount of DNA and protein sequencing data on tomato has been accumulated. Recently, the number of tomato genome sequences in the International Nucleotide Sequence Databases has been rapidly increasing due to ...
متن کامل